Results of my MTT assay.

Hey guys ive been talking alot on MCT stuff and MTT assay. Now its time for me to show the fruits of my labour...........the RESULTS!!!

Cell Sensitivity (MTT) assay

Cool ah?? Hahha. With this result, i will find the IC50 of the drug against the Hep G2 cells.

IC50 or the half maximal inhibitory concentration, represents the concentration of the inhibitor (drug) that is required for 50% inhibition of its target (Hep G2 cells). IC50 measures how much the drug is required for 50% inhibition in-vitro.

I will take a minimum of 5 points to form a linear line. Then using Microsoft Excel, i will obtain the equation of line and calculate the IC50.








With the equation, substitute the y value with 50 and then the x value could be obtained and that is the IC 50 of drug towards Hep G2 cells.

Enlightened?? Hahhaha See you guys in sch.


Najib Bin Hamid
(0503217B)