Preparation of a Hanging Drop

this is a simple experiment to observe the movement of microorganisms under microscope.

Materials:
- bacteria sample
-cover slip
-glass slides
-plasticine
-inoculating loop
-microscope

Method:

1. Use plasticine provided; make a loop about the size of a 5 cent coin.
2. Place the loop firmly onto the center of a clear glass slide.
3. Aseptically transfer a loop of sample broth culture onto the center of the clean cover slip.
4. Place the glass slide (with the plasticine loop facing downwards) over the cover slip and turn the glass slide over. The drop of culture must appear to hang down from cover slip.
5. View 10X and 40X

Movement of E. Coli was observed. i thought the E. Coli will be swimming, but actually movement of E. Coli under microscope is just vibrating in the original position.

Difficulty faced during experiment is when i was expected to turn the glass slide over, the E. Coli tends to slide to the side of the plasticine. it was only after a few tried that i finally managed to do it right.

that's all!

Suat Fang
0503328G