back to me agn (:

ouh. when i am blogging, it means that 2months of sip have passed!
how time flies eh?

Ha. Anyway back to serious business.
Yes today i shall post bout the experience i had and what i've learnt in the Mircobiology lab.
A short brief one that is. (=
Basically the microlab consists of 5 benches; namely,
1)Specimen Processing Bench; whereby all the specimens are being processed and sorted out.
2)Urine Bench; whereby necessary interpretation of primary urine culture plates(that are cultured the day before) and performing of tests for all positive tests.
3)Microscopy Bench; its whereby the staff in-charge process specimens such as sputum for diagnosis of TB/myobacteria infection.
4)Blood Bench; its the same process as in the urine bench, just that the specimen is of blood.
5)Miscellanous Bench; as its name called, its of interpretation and culturing of other specimens than blood and urine.

I shall focus more on the Bacteria ID Identification procedure. Its rather interesting, the best part i liked! and its bascially like the die-die-must-know thing in a mircolab. lol

First, gram-staining is done on the unknown bacteria.
Then by using the microscopy lens, identify the bact is of a gram positive (violet colour) or a gram-neg(pink colour).

For the gram-pos bacteria,
a Catalase test is done.
If its catalase positive- it is a Staphylococcus bacteria.
A Coagulate (latex) test is done to further identify its type.
So if its catalase pos, it is either a Stap.aureus or MRSA.
If its a catalase neg, it will be reported as STCH (normal commensal flora).
For catalase neg- it is a Streptococcal organism. Further tests e.g. alpha test, beta-grouping test etc are being carried out. Normally, the beta-grouping method is the most common, it tell us what group type it belongs to.

For gram-neg bacteria,
we'll carry out the biochemical tests (i.e. 7tubes of different tests + F12)
F12 is of antimicrobial agents such as penicillin, it tells us the sensitiveness and susceptibility of the bacteria towards different types of antibodies.
Biochemical Tests are of the followings:
1)KIA slant
2)Simmons citrate
3)Urease
4)Motility (& OF) test
5)Indole
6)Phenylalanine slant
7)Malonate test

So, by identifying the positive or negative results of the various tests will help us to differeniate which Enterobacteriaceae group it belongs to!

okay i am done with all the brief short explaination.
If you guys need futher explaination on the principles and the result interpretation of the various biochemical tests, just give me a message! i'll post it asap okay.

Have fun SIP-ing guys/babes. (:


signed,
Natalie